Composite

Part:BBa_K5250021

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


pT7-like + GFP

GFP is expressed after activation of the T7-like promoter (BBa_K5250003) with a T7-like RNA polymerase (BBa_K5250002) originating from a Pseudomonas phage.

This transcriptional unit is part of the plasmids we are using to test the Feedback loop in our construct. The expression of GFP is to help us quantify the activity of the T7-like promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 131
    Illegal AgeI site found at 592
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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